The TECHNOCHROM® FVIII:C is a kit for the determination of coagulation factor VIII activity in plasma and factor VIII concentrates by measuring factor Xa generation with a chromogenic substrate. The kit shows an excellent correlation with one and two stage Factor VIII assays and a linear calibration curve between 0 and 130%. It contains all components and is insensitive to heparin up to 10 IU/mL.
FVIIIsample + (FIXaß-PL- Ca++) ————u FVIIIa-FIXaß-PL-Ca++-complex
F VIIIa-F IXaß-PL-Ca++-complex
F X ————————————————u FXa
Substrate-FXa-1 ——————u Substrate-FXa-1 + pNA